The MyTaq One-Step RT-PCR Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube. A combination of the latest advances in buffer chemistry, together with a reverse transcriptase and hot-start DNA polymerase system ensure fast, highly-specific and ultra-sensitive one-step RT-PCR, from either total RNA or mRNA using gene-specific primers.
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The MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and a potent RNase Inhibitor, RiboSafe, that collectively create a simple to use all-in-one mix. The kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative, semi-quantitative or quantitative analysis of RNA transcription levels. The one-step format is also perfect for the synthesis of double-stranded cDNA products for subsequent gene-expression analysis.
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