SensiFAST One-Step qPCR Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system, ensures that SensiFAST One-Step Kits produce fast, highly-specific and ultra-sensitive one-step real-time RT-PCR.
SensiFAST One-Step qPCR Kits have been developed for fast RT-qPCR and has been validated on all commonly used real-time instruments. They are available either as SYBR or Probe mixes. The antibody-mediated hot-start DNA polymerase system reduces the chances of primer/dimers formation, allowing a much greater dynamic range by removing competition for reaction components during amplification, leading to greater sensitivity.