TRIsure is a ready-to-use reagent, for the isolation of separate fractions of RNA, DNA and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.
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TRIsure allows the user to perform sequential precipitation of RNA, DNA and proteins from a single sample. After homogenizing the sample with TRIsure, the homogenate is allowed to separate into three phases: an aqueous phase (upper), an organic phase (lower) and an interphase. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation. The highly effective RNase inhibitory property of TRIsure protects the integrity of the RNA during lysis and results in the isolation of high-quality material. DNA is precipitated from the organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation.
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