Quick-Stick Ligase

Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room temperature. Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to improve enzyme activity, and contains a specially developed 4x Quick-Stick Buffer. The enzyme catalyses the joining of two strands of DNA between the 5’-phosphate and the 3’-hydroxyl groups of adjacent nucleotides in either a blunt-ended or cohesive-ended configuration. Quick-Stick Ligase will ligate 99% of λ/HindIII cohesive-ended fragments, or 80% of λ/EcoRV blunt-ended fragments, in 5 minutes at room temperature. 100% ligation of blunt-ended fragments can be achieved by increasing the ligation time to 15 minutes at room temperature.

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